Are sons of Lewites (Levites) determined by the Father's lineage or Mother's lineage?

Answer From The Annual Reading Schedule - Week 32 Notes For Numbers: Father's lineage. We never see that the Mother's lineage is used to determine what tribe anyone is from. A good example is Numbers 4:34 which says the Qehathites (Kohathites) were registered by their Fathers' house. A woman's tribe changes the moment she marries outside of her tribe, so it wouldn't make sense to determine tribe based upon the woman.

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